Novel cell model | human immortalized neonatal melanocytes
Background 1. Neonatal dermal melanocytes are pigment-producing cells. 2. Skin pigmentation is a multi-dimensional and complex process; Melanin, produced in melanocytes, is packaged in melanocytes, then exocytosis out of the cell and endocytosis by adjacent keratinocytes. 3. Many genes play a role in controlling pigmentation and melanin production at different levels. 4. These genetic mutations are characteristic of a variety of skin disorders, including hyperpigmentation, hypopigmentation, and mixed hyperpigmentation/hypopigmentation. 5. In addition, external factors secreted by other suhhounding intrinsic cells also regulate the expression of melanin in melanocytes. 6. Human primary cells can serve as useful models to elucidate the biology of melanocytes and their toxicity to skin agents. However, primary cells have their limitations, such as donor variability and limited lifespan. Therefore, researchers need a more robust model system of human melanocytes to study skin pigmentation and the factors that affect it. 02 Solution Developed the human immortalized neonatal skin melanin cell line according to customer requirements: CRL-40641, established the human immortalized neonatal skin melanin cell line by inserting the overexpressed human telomerase reverse transcriptase (hTERT) protein into the primary neonatal skin melanin cell (ATCC®PCS-200-012™). 2. This newly established cell line can be used as in vitro toxicity and pigment for human immortalized dermal melanin cell line (ATCC®CRL-4059™)
2024-06-19